???????????? ??????? ? ?????????

[Xiren]

??????? ???????? ?? ?????? R. Schubbert, U. Hohlweg, D. Renz, W. Doerfler
"On the fate of orally ingested foreign DNA in mice: chromosomal
association and placental transmission to the fetus" Mol Gen Genet (1998) 259:569-576.
???????? ?????? ??? ??? ???? ???????.

??? ???, ??? ?? ????? ??????: ? ???? ?????????? ????? ????? ????????? ???????? ? ????? ??????????????? ???????? ????? (plasmid containing the gene for the green fluorescent protein (pEGFP-C1).

???????, ??? ?????????? ?????. ???? ????????? ???????? c ????????? ?????????????? www.translate.ru

??? ??? ???????? Schubbert ? ?????.:

The ingested foreign DNA can transgress not only the intestinal but also the placental barrier and reach the nuclei of cells of fetuses or newborns from female mice that have been fed the test DNA daily for longer periods during pregnancy. FISH (Fig. 2) and PCR data (Fig. 3) reveal the presence of the test DNA in the nuclei of clusters of cells in the fetus or in newborn mice. Some of this DNA is associated with chromosomes (Fig. 2g), and is probably integrated into host DNA. Chromosomal association of the orally ingested test DNA with both chromatids could be demonstrated in rare instances.

The intimate association of the foreign test DNA with both chromatids in cells of the offspring and the long-term persistence of foreign DNA in newborn animals for up to 3 months after birth raise novel questions about the genetic effects that this intrusion might have on the fetus.
It will be a challenging task to investigate the possible consequences of foreign DNA insertion into the genomes of the recipient organisms.

[gm]

Xiren, ?? ??????????? ?? ?? ???????? ?? ???????! ????? ???? ???? ? ?????? ??????, ?? ??? ?? ????? ?????? ?????? ??????? ?????????. ?? ?? ???????? ????, ????? ???-?? ????? ??? ???? ?????? ??..!

????, ??????? ????????, ??? ?? ?? ??? ?????????????.
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http://www.gmo.ru/forum/showpost.php?p=259&postcount=12
http://www.gmo.ru/forum/showpost.php?p=658&postcount=39
http://www.gmo.ru/forum/showpost.php?p=749&postcount=70
http://www.gmo.ru/forum/showpost.php?p=1569&postcount=83
http://www.gmo.ru/forum/showpost.php?p=1498&postcount=56

.
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??, ??????...
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FISH (Fig. 2) and PCR data (Fig. 3) reveal the presence of the test DNA in the nuclei of clusters of cells in the fetus or in newborn mice
?????? FISH ? PCR ??????????????? ? ??????????? ???????? ???. ? ???? ?? ???? ????? ??????????? ??? ?????????, ??? ??? ?????? ????????????? ?????. ? ??? ?????????? ?? ????????, ??? ??? ?????????? ? ?????????.

Some of this DNA is associated with chromosomes (Fig. 2g),
????????? ?????????? ??? ????????????? ? ??????????? - ????? ?? ?? ??????, ??? ??? ??????????. ? ???? ??, ?????? ????? ??? ??????? FISH. ?????, ??, ??????? ??, ?????? ??? ???????? FISH? ???? ????? ??????, ?? ????????? ????????? ???????: ????????????? ??????? ???-?????? ???????????? ? ??????????? ??????? ???????????? ???????. ??? ???? ??? ????????? ????? ?????????????? ???????????? ? ???????-?????????????? ? ????? ???, ? ??? ?????? ??????????, ??? ?????????. ?????????, ??? ??? ???? ?? ???? ????????? ?? ???????? ? ???????????? ????????.

? ?????? ????? ???????:
and is probably integrated into host DNA.
...? ???????? ????????????? ? ??? ???????. ?? ?????????? ????????! ?.?., ??? ???. ?????? ? ???? ?? ?????? ? ?? ???? ????? ????????, ? ??, Xiren, ??????? ? ???????????? ??? ??? ??? ?????? ? ????????? ?????????!!!

??? ????? ??????? ?????? ?????? ? ??????? ??? ??????????????? ??????? ???????? ??? ?? ???????? ?? ?????? ????????? ????? - ????? ? ?????????. ? ?????? ??? ? ??? ????????? ??? ?? ?????, ??? ?????? ?????...

Chromosomal association of the orally ingested test DNA with both chromatids could be demonstrated in rare instances.
????? ??, ????? ??? ??????????, ? ?? ??? ????????????... ?????????? ???????? ???? ???, ?? ? ????????? ????????? ???????: ?????????? - ??? ????????. ???? "???-?? ?????? ????? ???????". ? ???????????? (??? ????????) - ????? ???????? ? ??????.

The intimate association of the foreign test DNA with both chromatids in cells of the offspring and the long-term persistence of foreign DNA in newborn animals for up to 3 months after birth raise novel questions about the genetic effects that this intrusion might have on the fetus.
????? ????????, ??? ??????????? ???? ????? ??? ????????? ??????? ? ???????????? ???????? ??????? ????? ????? ?????. ??? ???: ????? ????? ?????! ?.?., ???. ?????? ????? ?? ??????, ? ?? ???????!! ? ??? ?????? ????????? ???????, ? ?? ???? ??????. ??????? ????????, ?? ?????? ???

It will be a challenging task to investigate the possible consequences of foreign DNA insertion into the genomes of the recipient organisms.
? ????? ????????, ??? ???????????? ????????? ??????????? ??????? ?????????? ??? ? ?????? ??????????-??????????? ????? ?????????? ???????. ? ???. ?? ????, ??????? ?????? ?????-?????? ? ??????? ??? ????????? (???? ?????????) ? ??? ???????...


??????, Xiren, ??????? ???????????? ?????????? ? ?? ??????????? ???? ?? ?????????. (????? ????? ???????????, ??? ????? ????, ????? ??? ?????.... :D ) ??? ??? ??? ??????? ???? ?? ?????????? ?????? ?????? - ??????? ?? ?? ????? ????...

[Xiren]

Gm ?????????? ???????? ??????. ???????? ?? ??? ??? "???????????" ????? ?? ????.
????? ? ??????? ??????? ? ??????????.

????? ? ???? ??? ?? 10? ?????, ? ???????, ???????????, ???? ???. ?? ???? ??? ?????? ???????????? ? ?????????? ??? ?? ????? ??????? ????????. ?? ??? ???????? ????????? ???, ??????? ????? ?? ????????????. ???????? ? ???????????? ????????? ???????? ? ????? ???????? ?????? ? ?????????? ?? ????? ?????????. ????? ??? ?????????????? ??????????? ??????? ????? ?????????? ? ??????? ?????? ??????? ????????.

? ???? ???????? ? ???, ????? ??? ??????? 50 ?????????? ??????? ??? ?????, ?? ????? ?????? ??????, ? ??????? ??????? ???????? ? ???????? ????????? ? ?????? ???????? ??-???????? ???? ????????, ??????? ???????? ? ??????????, ?.?. ?????? ?????. ??????? ? ????????? ???????? ? ???????? ???????? ???? ?????????????? ?????. ?????? ????? ???? ??? ????? ? ?.?. ??????? ?????? ?????????? ????? ??????????.
? ????? ??????????, ? ?????? ???? ???? ????? ?????????, ??? ?????? ????????? ??????? ? ??????? ???, ??? ?????? ???? ?????????? ?? ???? ? ?????????? ? ??? ??????, ??????? ???, ??? ????????? ???????????? ?????? ?????????? ???????? ????????????????? ????????? ???????? ??? ?????? ???????????. ? ??? ??? ?????? ?? ???????? ??????? ????? ?? ?????. ??? ?? ?????????, ?? ??? ?? ??????! ? ???? ????? ???-?? ????????? ??????????? ? ???????? ??????????? ???????, ??? ??? ???? ????? ?? ?????? ??????, ??????, ???? ?? ?????.
?? ?????? ?? ???????, ?? ? ???????? ????? ??????? ?????? ??????.

[BlackRaven]

<? ????? ??????????, ? ?????? ???? ???? ????? ?????????, ??? ?????? ????????? ??????? ? ??????? ???, ??? ?????? ???? ?????????? ?? ???? ? ??????????>
? ??? ???? ? ???, ??? ???????? (? ???? ????????? ????????) ???????? ? ???????? ???????? ?????????, ??????????? ??? ???????? ????

[gm]

Gm ?????????? ???????? ??????. ???????? ?? ??? ??? "???????????" ????? ?? ????.
????? ?????. ? ?? ????? ???????? ??????????....

????? ? ???? ??? ?? 10? ?????, ? ???????, ???????????, ???? ???. ?? ???? ??? ?????? ???????????? ? ?????????? ??? ?? ????? ??????? ????????.
?? ?????, ???? ??? ?????????? ? ???????? ?????? ???????. ? ???? ??????? ? ????????? ????????, ??, ? ???? ?????? ???, ??? ?????????, "?????? ????? ????????" (c). ?? ?????????, ??? 50??? ?????????? ??? ???? ??????? ?? ??? ???? ????? ?? ???????? ? ? ??????? ??? ?????????? ?????????? ????? ??????? ?? ????.

?? ??? ???????? ????????? ???, ??????? ????? ?? ????????????.
????. ??? ??? ??? ??????? ???????? ????? ???? ? ??? ?? ?? ???? ?????.
? ? ????? ??? ????? ??? ???, ? ???????, ??? ???????? ??????? ???????????? ??????????????, ????? ??? ??, ??? ? ????? ?????? ???. ?? ????????? ?? ?????? ??????? ??????? ? ???????? ??????????? ?????????? ? ???????? ???,
????? ????? ????. ???????? ????????? ??????, ??? ???? ?????? ???????? ??? ? ??????????? ?? ????? - ? ??? ?? ?????? ????????! ? ??? ??? ? in vivo...

???????? ? ???????????? ????????? ???????? ? ????? ???????? ??????
??, ??? ?????????! ? ? ??????? ???, ?????? ?? ????????...?

????? ??? ?????????????? ??????????? ??????? ????? ?????????? ? ??????? ?????? ??????? ????????.
????? ? ????????, ??? ?? ?? ????????? ??????? ????? ??????????? ???????? ??? ? ?????-???? ????????????? ????????, ? ???????? ???. ? ???, ????????, ??????? ???????. ?????? ??????????? ??? ?????? "??????????? ???????", ?? ?????????? ? ?????...
? ??????? ??? ????? ?????????? ???????? ??????? ???????????? ???????? ??? ?????. ???? ??? ???? ??? ????, ???? ??????????, ??? ?????? ??????.

? ???? ???????? ? ???, ????? ??? ??????? 50 ?????????? ??????? ??? ?????, ?? ????? ?????? ??????, ? ??????? ??????? ???????? ? ???????? ?????????
??, ??? ???? ???????? ? ???, ????? ??? ??????? 50??? ???????? ??? ?????? ?? ???? ??? ????????

? ?????? ???????? ??-???????? ???? ????????,
????. ??????????? ??????? ? ?????????? ???????? ??-????????. ???? ???? ???????? ??????? ????????, ?? ??? ?????????? ?? ??????, ??? ??? ????? ? ???? ???????? ? ? ?????? ????????.

??????? ???????? ? ??????????, ?.?. ?????? ?????. ??????? ? ????????? ???????? ? ???????? ???????? ???? ?????????????? ?????.
????? ??? ??? ??????, ??? ?????, ??? ????????????? ?? ?? ?????????? ? ??? ??????????, ?????? ???????... ? ?? ???-?? ?????????? ??????????. ?????? ?????? ??? ??? ?????????????? ??????? ?? ????, ?? ? ??? ?????, ??? ??? ????????. ?? ????? ??????? ??? ???? ??????? ????? ? ????????? ???????? ??????????.


?????? ????? ???? ??? ????? ? ?.?. ??????? ?????? ?????????? ????? ??????????.
? ???? ???! ????? ??????! ? ??? ?? ??????? ???? ???? ? ?????? ???? ? ???????? ? ?????? ????????..?

? ????? ??????????, ? ?????? ???? ???? ????? ?????????,
????? ??????????? ????? ?? ?????? ????????. ? ?????? ?? ??????, ??? ??? ??????? ?? ???.

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??? ??????? ?????? ???????

??? ?????? ???? ?????????? ?? ???? ? ??????????
??? ???? ?? ???, ????? ? ?????. ??????????, ??????, ???? ????? ??????!

? ??? ??????, ??????? ???, ??? ????????? ???????????? ?????? ?????????? ???????? ????????????????? ????????? ???????? ??? ?????? ???????????. ? ??? ??? ?????? ?? ???????? ??????? ????? ?? ?????.
???? ??? ???????. ?? ??????????, ? ????

? ???? ????? ???-?? ????????? ??????????? ? ???????? ??????????? ???????, ??? ??? ???? ????? ?? ?????? ??????, ??????, ???? ?? ?????.
?? ?????? ?? ???????, ?? ? ???????? ????? ??????? ?????? ??????.
?? ??? ???????, ? ??? ???? ???? ??! ??, Xiren, ??????? ? ??????????? ????? ?? ??????????? ???????, ????????!

[Xiren]

? ??? ???? ? ???, ??? ???????? (? ???? ????????? ????????) ???????? ? ???????? ???????? ?????????, ??????????? ??? ???????? ????

Raven, ???? ?? ????? ??????, ?? ????????? ???, ??????????, ?????? ??? ???????? ??? ???????????? ???????? ????????? ????????????????? ???????? ? ??? ??? ?????????? ?? ??????? ?????? ?????????

[Xiren]

????? ????? ????. ???????? ????????? ??????, ??? ???? ?????? ???????? ??? ? ??????????? ?? ????? - ? ??? ?? ?????? ????????! ? ??? ??? ? in vivo...

Gm, ??????? ?? ???? ?? ? ???? ?????????. ????? ???????? ???????, ??????? ??????????.
? ??? ???????? ? ???? "??????", ??? ?? ????????? ?? ??, ??? ? ??????. ??, ???, ????????, ? ???? ?? ?????. ?? ?????? ????? ????? ????? ??????.

[gm]

Gm, ??????? ?? ???? ?? ? ???? ?????????. ????? ???????? ???????, ??????? ??????????.
? ??? ???????? ? ???? "??????", ??? ?? ????????? ?? ??, ??? ? ??????. ??, ???, ????????, ? ???? ?? ?????. ?? ?????? ????? ????? ????? ??????.
??-??????, ? ? ???? ???? ?? ?????????? ?? ???. ??-??????, ? ??? ????? ?????????? ??????? ?? ????????, ????? ?? ???????? ??????? ??????.

[Xiren]

??-??????, ? ? ???? ???? ?? ?????????? ?? ???. ??-??????, ? ??? ????? ?????????? ??????? ?? ????????, ????? ?? ???????? ??????? ??????.

????????? ????, ?????? ?? ?? ?? ?????????????. ?? ?????????? ????? ??????, ?????? ?? ? ????????. ????? ???? ????? ?????? ???? ??????

[dimi4]

?? ?????? ????????? ? ?????????? ??????? ? ??????????????? ??????????:

???? ?? ??? ??? ???? ??? ?? ???????????, ?.?. ????? ????? ????????????? ? ?????????????????? ?????????? ??? ? ????? ?????? ??????????? ???????? (??? ????????) ?? ??? ???, ??????? ???? ? ??? ??????, ???????? ?? ?? ????????????. ? ????????? ?????????? ?? ???????? ?? ???????????? ?????? ???????? ???????. ?? ???? ?? ????? ?????????? ??? ????????????? ?????? ??????????? ????????? ? ??? ?????.

?? ???????:

?????? ?? ????? ??? ????????????? ????? ??????????? ??????? ??? ??????????? ???????? ?????????? ??? ? ????? ???? ??? ?? inverse PCR, ? ?? FISH. ??? ??????? ?? ????, ?? ?????, ?? 10 ??? ??????.

[Xiren]

?? ?????? ????????? ? ?????????? ??????? ? ??????????????? ??????????:

???? ?? ??? ??? ???? ??? ?? ???????????, ?.?. ????? ????? ????????????? ? ?????????????????? ?????????? ??? ? ????? ?????? ??????????? ???????? (??? ????????) ?? ??? ???, ??????? ???? ? ??? ??????, ???????? ?? ?? ????????????. ? ????????? ?????????? ?? ???????? ?? ???????????? ?????? ???????? ???????. ?? ???? ?? ????? ?????????? ??? ????????????? ?????? ??????????? ????????? ? ??? ?????..

????? ???????. ????? ?????? ????????? ???????

?? ???????:

?????? ?? ????? ??? ????????????? ????? ??????????? ??????? ??? ??????????? ???????? ?????????? ??? ? ????? ???? ??? ?? inverse PCR, ? ?? FISH. ??? ??????? ?? ????, ?? ?????, ?? 10 ??? ??????.

? ????? ??????? PCR ?? ?????????

[dimi4]

.

????? ???????. ????? ?????? ????????? ???????

?? ???????:



? ????? ??????? PCR ?? ?????????

??? ?????? ? ??????? ???????? ? ??????????? ??????? ????????? ??? ??????????? ????????? ?? ???????

PCR ?????????? ??????? ??? ??? ???? ??? ? ???????. ?? ?????. ???????? ??? ??? ????-?? ??? ??? PCR ?? ???????

[gm]

?? ?????????? ????? ??????, ?????? ?? ? ????????. ????? ???? ????? ?????? ???? ??????
?, ? ?????-??, ? ?? ??????.

[Eraser]

? ???? ???? ?????????????????? ??????????? - ????????? ??????????? FAQ ?? ???????????? ????????????? ???????, ??????? ?????? ?? ????? ????? ?????? ? ??????, ? ??????????? ?????? ?? ???. ??????????? ?? ???????? ?????????? ? ???????? ?? ?????, ? ??????? ???????? ???? ???????????.
????? ????? ????? ? ????? ?? ???????????? "?????? ???????..." ?????? ???????? "?????? 3, 15, 24". :)
??????? ????? ??? ??? ?????? ??? ?????? ????? ???????

[Biologist]

??????, ? ???? ??? ?? ?????? ?? ? ???? ????? ?? ?? ???? ?? ??????, ? ?????????, ??? ?????, ??? ... ???? ?? ?????? ???????? ???????

[Eraser]

Biologist, ?? ?? ???? ? ???????? ?????? ?? ? ???? ????, ??? ?? ?? ??????, ?? ??? ?? ?????? ?????? ?? ????? :) ??? ?, ???????????, ?????
? ????? ??? ? ????? ?? ????? ????????????? ?? ???? ??????, ? gm'?? ????????, ? ??????? ??????? ?? ? ???, ??? ????? ???? ???????? ??? ???? :D

[Xiren]

?, ? ?????-??, ? ?? ??????.

??. ????????? ???????????? ? ??????? ????? ?????? ?????????.